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Thread: Effects of Media on Micro-Propagation of Grapevine (Vitis L.)

Created on: 04/16/10 08:23 PM

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mec8626





Joined: 04/15/10

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Effects of Media on Micro-Propagation of Grapevine (Vitis L.)
04/16/10 8:23 PM
Attachment: Research Abstract -Melissa2.doc

Melissa Cadet*, Xia Xu, Jiang Lu

Center for Viticulture and Small Fruit Research
College of Engineering Sciences, Technology and Agriculture
Florida A&M University
Tallahassee, Fl 32304
*Presenting Author

Abstract:
In-vitro propagation is a rapid way of obtaining multiple plants in a short period of time. It is used to multiply plant that have been genetically modified or bred through the method of plant breeding. The process begins with a selection of the plant material that is going to be propagated in a selective growth medium. A growth medium typically consists of mineral salts, sucrose as an energy source, plant growth regulators (plant hormones), and agar; which are used to gel thicken the medium in order to support the plant. Some plants require growing on specific media for successful growth. An experiment was conducted to analyze the medium effects on plant growth of muscadine grape (V. rotundifolia) cultivar ‘Alachua’ and bunch grape (V. vinifera) ‘Thompson Seedless’ in order to find the best medium for grapevine propagation. Two media, Woody Plant Medium (WPM) and Woody Plant Medium plus 1 µm/l BA (6-benzylaminopurine), were used for this study. Two months after the culture, plants grew much better on WPM+1 µm BA than on the basic WPM, particularly the bunch grape cultivar ‘Thompson Seedless’. This result indicates that addition of moderate amount of plant growth regulator BA on WPM promotes grapevine growth. Medium WPM + 1 µm BA was the best medium for in-vitro grapevine propagation.

Key Words: Woody Plant Media, in-vitro propagation, Grapevine


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