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Thread: Determination of Fungal Pathogens of the Ectoparasitic Mite, Varroa destructor (Acari: Varroidae)

Created on: 04/15/10 10:53 PM

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eddinco





Joined: 04/15/10

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Molecular Identification of Fungal Pathogens of th
04/15/10 10:53 PM
Attachment: ABSTRACT_2-17-f[1]revised.doc

Molecular Identification of Fungal Pathogens of the Ectoparasitic Mite, Varroa destructor (Acari: Varroidae) of Honey Bee, Apis mellifera (Hymenoptera: Apidae)

Courtnee Eddington1* and L. H. B. Kanga1

1Florida A&M University- College of Engineering Sciences, Technology and Agriculture - Entomology Program – Tallahassee, FL 32307

*Presenting Author

This study will be carried out to determine if mortality of Varroa mite is due to fungal infections. Thus, cadavers of Varroa mite will be collected from an Apiary located in Quincy, FL and brought to the laboratory in Tallahassee, FL. The infected cadavers will be surface-sterilized by immersing them in a sterilant disinfectant, Expor for 3 minutes and rinsed with 95% ethanol for 2 minutes. The cadavers will then be plated on Petri dishes containing Sabouraud maltose agar supplemented with 1% yeast extract and incubated at 27 ± 1°C, 85 % RH, and 13:11 (L:D) h photoperiod. The Petri dishes will be sealed with parafilm prior to incubation and dead mites will be observed daily for the presence of external fungal hyphae.
Only mites that show fungal growth will be considered to have died of infection. The fungus will then be isolated and cultured on new Petri dishes for use in DNA fingerprinting techniques for identification.

Keywords: Varroa mite, infected cadavers, surface-sterilized, fungal growth, DNA fingerprinting techniques
* Last updated by: eddinco on 4/16/2010 @ 8:43 AM *


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