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Thread: INDUCTIVE RESPONSE SUBJECTED TO PLANT GROWTH REGULATORS IN ANTHER CULTURE OF VITIS SP.

Created on: 04/15/10 12:17 PM

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TiffanyKerr





Joined: 04/15/10

Posts: 2

INDUCTIVE RESPONSE SUBJECTED TO PLANT GROWTH REGULATORS IN ANTHER CULTURE OF VITIS SP.
04/15/10 12:17 PM
Attachment: Research Abstract_Ms. Tiffany Kerr_Colova-Spring 2010.doc

Somatic embrogenesis (SE) can be induced in vitro from various plants’ tissues, both generative and vegetative origin. Anthers are the preferred generative explant in grape SE initiation. Embryogenic cells cultures are widely used for micropropagation on a large scale for in vitro selection and various functional genomics and gene transformation technologies. The objective of this study is the SE initiation in anther culture of the grape rootstock variety “3309 Couderc” for purpose of gene transformation as a model system for gene expresion. Single node dormant cuttings ware used to force inflorences out of season under greenhouse conditions in order to isolate the anthers and to induce embrogentic callus. Somatic embryos from anthers were obtained after exposure of the explants to 2.4 D/BAP growth regulators. A process of dedifferentiation, cell division and embryogenesis were observed. Embryogenic lines will be maintained both as clusters of differentiated somatic embryos through secondary embryogenesis on solid medium and as proliferative Polyemryogenic Masses (PEMs) in liquid cell suspension culture. High frequency of conversion of PEMs to regenerated grape plantlets is expected to be achieved under the effect of the dehydration of the culture and growth regulator treatments.

Key words: grape, somatic embryogenesis, regeneration, Vitis


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